Infrared spectroscopy has been increasingly used to characterize biological matrices, providing a straightforward method to obtain diagnostic and observational information from easily acquired samples. This enables the monitoring of modifications and ultimately characterizes a biological matrix to detect early alterations that can lead to disease. Infrared spectroscopy it is becoming more and more useful in the field of biomedical research. The potential advantages of using IR spectroscopy of biofluids or tissues for infectious disease detection include the following: no reagents are required, a profile of spectral alterations can be determined, and the methods are suitable for automation. Sample preparation is minimal, techniques involved are relatively low cost, and data frameworks are available. This presentation tries to review some of the most relevant data related to infectious disease diagnostics using infrared spectroscopy published after 2020.