Mimosa pudica is a traditional medical plant. In the present investigation, antibacterial activity of ethanolic extract of mimosa pudica was studied. The antibacterial activity was assessed by agar-well diffusion method by using Muller-Hinton agar medium. The zone of inhibition was compared with the standard antibiotic Gentamycin. The zone of inhibition of fraction was comparable with Gentamycin. 8 mg/mL concentrated mimosa pudica plant extract shows near activity to the standard drug Gentamycin. The finding confirms that the traditional therapeutic claim for this plant in near future will surely be able to replace the conventional anti-bacterial agent, to which there is increased incidence of drug interactions and the study suggests that this plant is promising for development of phytomedicine for antibacterial properties